So what is the exact order from start to finish on tuning with the machineheads? And how many times should I need to do this?
Ibanez RG7321
Jackson Randy Rhoads V with Floyd Rose
Peavey Valveking 112
Digitech RP70 Guitar Processor
until you are as close as u can get without spending a ton of time.

then lock em down, fine tune at the bottom and you're set!
I like to cross tune. Tune the low E, then the A, the the low E again, then the D, then the A, then the low E, then the G...and so on and so forth.

At the end I fine tune it after locking the nut.