#2
Hi, I see this is your first post so... Welcome to Ultimate Guitar!

To tune the Low-E String, you must adjust your tuning key, just like the other strings, and tune it to the Low-E note.

Here is a Youtube video with reference pitches, and their respective strings!

http://www.youtube.com/watch?v=nTzcyASaQ40&feature=related

Just tune your guitar to the pitches shown!

Hope This Helps!
Gear:
Fender FSR Standard Stratocaster SSS (MIM Gilmour Black Strat) -
Agile AL-2000 CSB -
Fender Super Champ XD -
Homemade Talkbox -
THE BORG COLLECTIVE