I have just bought a BOSS TU-15 chromatic tuner and I am going to do the intonation on my Les Paul.

I'm not quite clear on one thing though: I know I should use new strings to do the intonation, but should I do it immediately after I put the strings on and stretch them? Or should I play it for a bit and then do the intonation after the strings have been used for an hour or so?

Thanks in advance
Does anyone remember laughter?

Manuel, please try to understand before one of us DIES.

my gear:

Gibson LP Standard
Epiphone SG
Classical guitar
Peavey ValveKing 112
Marshall MG15